Engineering triterpene production in Saccharomyces cerevisiae-β-amyrin synthase from Artemisia annua
نویسندگان
چکیده
منابع مشابه
β‐Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae
Conyza blinii H.Lév. is a widely used medicinal herb in southwestern China. The main pharmacological components of C. blinii are a class of oleanane-type pentacyclic triterpene glycosides known as conyzasaponins, which are thought to be synthesized from β-amyrin. However, no genes involved in the conyzasaponin pathway have previously been identified. Here, we identify an oxidosqualene cyclase (...
متن کاملArtemisia annua L
Shoot cultures of Artemisia annua L. were cultivated in three different micropropagation systems: an ultrasonic nutrient mist bioreactor (UNMB), a modified ultrasonic nutrient mist bioreactor (MUNMB) and solid culture in Magenta boxes. The shoots cultivated in the UNMB and MUNMB showed excellent growth. The dry weight increase (35 times) of shoot cultures in the MUNMB was higher than those (25 ...
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In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and ...
متن کاملSaponin Biosynthesis in Saponaria vaccaria. cDNAs Encoding b-Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase
Saponaria vaccaria (Caryophyllaceae), a soapwort, known in western Canada as cowcockle, contains bioactive oleanane-type saponins similar to those found in soapbark tree (Quillaja saponaria; Rosaceae). To improve our understanding of the biosynthesis of these saponins, a combined polymerase chain reaction and expressed sequence tag approach was taken to identify the genes involved. A cDNA encod...
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Inactivation of TPI1, the Saccharomyces cerevisiae structural gene encoding triose phosphate isomerase, completely eliminates growth on glucose as the sole carbon source. In tpi1-null mutants, intracellular accumulation of dihydroxyacetone phosphate might be prevented if the cytosolic NADH generated in glycolysis by glyceraldehyde-3-phosphate dehydrogenase were quantitatively used to reduce dih...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2008
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2008.06343.x